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DICONO DI NOI
JOINT RESEARCH AGREEMENT BETWEEN “Istituto Sperimentale per l’Orticoltura” AND “Four-Ortoricerca” TO IMPROVE SOME TOMATO ITALIAN ECOTYPES.
N. Acciarri; G. Vitelli; G. Campanelli; E. Sabatini;
(Istituto Sperimentale per l’Orticoltura Sezione di Ascoli Piceno, Via Salaria 1 Monsampolo del Tronto AP. e-mail: acciarri@libero.it)
P. Sbarbati
(Four-Ortoricerca)
Former italian ecotypes
The old selections (Figure 1), carried out after the discover of tomato nutritional value, gave rise to original ecotypes, with different names, but with shape and features sometimes similar: : “San Marzano”, “Riccio di Panocchia”, “Scatolone di Bolsena”, “Pantano Romanesco”, “Pera d’Abruzzo”, “Cuor di bue ligure” or “Cuore di Albenga” or “Pera ligure”, “Pizzutello”, “Principe Borghese”, “Rosa di Rotonda” or “di Sorrento”, ecc.
New and better-defined F1.hybrids have slowly taken the place of the former cultivars, nowadays nearly disappeared, except for those still grown in small areas for local usages and consumption.

A renewed interests for traditional products
It possible to find some particular ecotypes in little local markets but now an interest by the large-scale retail trade exists. Particularly, “Ligure” type is grown not only in open field but also in Sardegna and Sicilia greenhouses (figure 3); furthermore, both Marche and Abruzzo regions are interested to give higher value to the local tomato ecotype “Pera d’Abruzzo”.
Despite the increasing interest toward this old tipologies of tomatoes, it is not possible to improve them with a normal pedigree selection, because of its narrow genetic base and the lack of important characters such as genetic resistences to diseases and physiological disorders. In order to retrieve this old cultivars for the large-scale retail market, hybridization with suitable pure lines is of high interest.
In this context, a joint program for the genetic improvement was started in 1999 by “Istituto Sperimentale per l’Orticoltura” and “Four-Ortoricerca” to obtain new lines and hybrids with the typical “Cuor di Bue Ligure” and “Pera d’Abruzzo” features coupled with superior firmness and genetic resistance to the main diseases.


Figure 1: Large variability in the “ISPORT” germplasm


Figure 2:fruit set in a F1 hybrid of “Cuor di Bue Ligure” typology.


Figure 3: improved fruit set in a F1 hybrid of “Cuore di Bue Ligure” typology grown in greenhouse (below)

Figure 4: typical “cracking” in the original ecotype of “Pera d’Abruzzo”.

Breeding activity
Ecotypes retrieving and costitution of lines useful for the breeding purposes.
The Istituto Sperimentale per l’Orticoltura (ISPORT) started, before the joint-agreement with “Four-Ortoricerca”, a breeding program in order to get a better knowledge of the main characteristic for each local ecotype of interest. In this way a wide germplasm has been collected and evaluated. With recurrent selection and backcrosses a series of segregant lines were produced, which showed an improved firmness of the fruit and especially resistance to important diseases such as Verticillium and Fusarium sp.
The new lines obtained during the breeding program were selected within the original ecotypes to uniform the variability found; lines with different dimension, shape, and color of fruit (with or without green shoulder), and able to grow both in open field or greenhouse condition were selected. “Four-Ortoricerca” as well, provided new original germplasm, in order to increase the variability.
The improvement for disease resistance was carried out using as starting materials the segregating progenies derived from the cross between the ecotypes, and actually diffused types provided with dominant genes of resistance.
During the three-years agreement both participants carried out a joint selection of segregant lines. The best local accession were selected to obtain parental lines and than F1 hybrids which maintain the typical characters of the original ecotypes.
Diallelic crosses were made to study the combining ability. The hybrids obtained were tested in agronomical trials in open field and in grennhouses located in different areas (Marche, Abruzzo, Sicilia, Sardegna, Liguria, Piemonte). The trials consisted in the evaluation of fruit quality and production under different agonomic conditions and treatments: normal open-field farming, greenhouse coltivation, use of rootstock resistant to many diseases, applications of phytormones to improve fruit set, use of bumble bees for pollination.
Generally, the F1 hybrids showed an improved fruit quality and the highest yield (Figure 5)

Figure 6: Greenhouse production of a F1 hybrid of “Cuor di Bue Ligure” type


Figure 5 a, b, c: results of some agronomical trials

Main results
At the end of this agreement, the “Istituto Sperimentale per l’Orticoltura” released 23 pure lines and 6 segregant lines to “Four-Ortoricerca”, with features belonging to the “Cuor di Bue Ligure”, “Rosa di Rotonda”, “Rosa di Sorrento”, “Pera d’Abruzzo” tipologies.
“ Four-Ortoricerca” started in 2003 the production of F1 hybrids, in order to realize larger evaluation

Figure 7: marketing solutions for a “Cuor di Bue Ligure” hybrid

Figure 8: high price of some “Cuor di Bue Ligure” tomatoes on the market
trials and to inscribe some of them to the National Register of Varieties. Many large-scale market operators were interested to these hybrids, using them for marketing trials, thus confirming a high interest from the consumers toward these new typologies of tomato (Figure 7 and 8).


nel mese di FEBBRAIO si seminano:

BASILICO
BASILICO (Vivaio)
BIETOLA DA ORTO E BARB.DA ORTO
BIETOLA DA COSTE
BIETOLA DA COSTE (Vivaio)
CAVOLO E CAVOLFIORE
CAVOLO E CAVOLFIORE (Vivaio)
CETRIOLO E CETRIOLINO
CETRIOLO E CETRIOLINO (Vivaio)
CIPOLLA
CIPOLLA (Vivaio)
COCOMERO O ANGURIA (Vivaio)
FAVA
FAGIOLO E FAGIOLINO
FAGIOLO E FAGIOLINO (Vivaio)
...
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